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KMID : 0043320070300010001
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Archives of Pharmacal Research
2007 Volume.30 No. 1 p.1 ~ p.7
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Antioxidant Constituents and a New Triterpenoid Glycoside from Flos Lonicerae
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Choi Chun-Whan
Jung Hyun-Ah
Kang Sam-Sik
Choi Jae-Sue
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Abstract
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As a component of our continuing investigations into herb-derived antioxidant agents, we have evaluated the antioxidant effects of Flos Lonicerae (Lonicera japonica flowers), via 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, total reactive oxygen species (ROS), hydroxyl radical (·OH), and peroxynitrite (ONOO?) assays. Among the methanolic extract and the dichloromethane, ethyl acetate, n-butanol, and water fractions, the EtOAc fraction of Flos Lonicerae exhibited marked scavenging/inhibitory activities, as follows: IC50 values of 4.37, 27.58 ± 0.71, 0.47 ± 0.05, and 12.13 ± 0.79 μg/mL in the DPPH, total ROS, ONOO?, and ·OH assays, respectively. Via a bioactivity-guided fractionation approach, a new triterpenoid glycoside, oleanolic acid 28-O-α-L-rhamnopyranosyl-(1→2)-[β-D-xylopyranosyl(1→6)]-β-D-glucopyranosyl ester (12), along with eleven known compounds, including chrysoeriol (1), luteolin (2), 5-hydroxymethyl-2-furfural (3), caffeic acid (4), protocatechuic acid (5), chrysoeriol 7-O-β-D-glucopyranoside (6), isorhamnetin 3-O-β-D-glucopyranoside (7), kaempferol 3-O-β-D-glucopyranoside (8), quercetin 3-O-β-D-glucopyranoside (9), hederagenin 3-O-α-L-arabinopyranoside (10), and luteolin 7-O-β-D-glucopyranoside (11), were isolated from the EtOAc fraction. The structures of isolated compounds 1-12 were elucidated via spectroscopic analyses. Compound 12 was isolated from a natural source for the first time. Compounds 2, 4, 5, 7, 9, and 11 evidenced marked scavenging activities, with IC50 values of 2.08-11.76 μM for DPPH radicals, and 1.47-6.98 μM for ONOO?.
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KEYWORD
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Flos Lonicerae, Lonicera japonica, Oleanolic acid glycoside, Flavonoids, Antioxidant activity
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